mRNA (messenger RNA) screening as candidate for vaccines.

CleaScrip™ Custom mRNA (Low dsRNA)

CleaScrip™ mRNA (via Gentaur) – Practical Advice​

• When to choose CleaScrip™
• 
  
• You work with immune-sensitive systems (primary cells, stem cells, in vivo models).
• 
  
• You need low innate immune activation (low dsRNA).
• 
  
• You want high translation efficiency without complex downstream cleanup.
• 
  
• What to clarify with Gentaur (before ordering)
• 
  
• Ask the Gentaur technical representative to review:
• 
  
• mRNA length and GC content (impacts yield and integrity)
• 
  
• Cap structure (Cap1 standard; alternatives if needed)
• 
  
• Poly(A) tail length (default ~100 nt; adjust for stability)
• 
  
• Modified bases (e.g. ψU, m5C for reduced immunogenicity)
• 
  
• Final concentration & buffer (for transfection or LNP prep)
• 
  
• QC package (dsRNA level, integrity, capping efficiency)
• 
  
• Design recommendations
• 
  
• Prefer codon optimization for mammalian expression.
• 
  
• Avoid strong secondary structures at the 5′ UTR.
• 
  
• For mRNA >5 kb, confirm expected integrity % in advance.
• 
  
• Logistics & support
• 
  
• Gentaur handles quotations, lead times, and ordering.
• 
  
• A Gentaur technical representative can visit your lab to:
• 
  
• Advise on experimental design
• 
  
• Compare CleaScrip™ to standard IVT options

1. Align specs with your delivery method (lipofection, LNP)
2. 
   
3. Common pitfalls to avoid
4. 
   
5. Ordering unmodified mRNA for immune-sensitive assays.
6. 
   
7. Not confirming dsRNA specifications in QC.
8. 
   
9. Underestimating storage and handling (RNase-free workflow, −80 °C storage).
10. 
    
11. Best use cases
12. 
    
13. Proof-of-concept expression studies
14. 
    
15. Preclinical mRNA workflows

Comparison studies vs standard T7 IVT

Top-notch mRNAs characterized by  Yeasen's low dsRNA levels and exceptional integrity are indispensable for disease research utilizing mRNA tools and the advancement of RNA therapeutics.

https://www.yeasenbio.com/pages/cleascrip%E2%84%A2-mrna-synthesis-low-dsrna

https://www.yeabio.com/pages/cleascrip%E2%84%A2-mrna-synthesis-low-dsrna

Due to the ability of dsRNA to signal through Toll-like receptors TLR3 and RLR-mediated pathways, it is crucial to control the dsRNA content during clinical research. In primary cells and stem cells, the presence of dsRNA-binding receptors will inevitably trigger an immune response. 

Yeasen offers exceptional control over the dsRNA content in mRNA.

In conventional mRNA synthesis services do not include a step for dsRNA removal. Adding this step increases costs, making the product more expensive. Typically, mRNA is categorized into RUO (Research Use Only) grade and preclinical grade, with dsRNA content being a key distinguishing factor. Yeasen Biotechnology, through collaboration with third parties and the use of the CleaScrip™ T7 RNA polymerase, can provide mRNA with ultra-low dsRNA content for drug or vaccine development scientists from the outset. This enables researchers to access near-clinical-grade quality mRNA at a lower cost from the very beginning.

Our exclusive mRNA manufacturing process relies on CleaScrip™ T7 RNA Polymerase, enabling the production of high-quality mRNAs with remarkably low dsRNA content (approximately 1/100000), coupled with numerous customizable features.

A Comprehensive Solution for CleaScrip™ mRNA Production

Our mRNA production service simplifies your process from protein sequence to LNP delivery. Our expert team guarantees the excellence and expression effectiveness of your mRNA, offering a seamless, all-in-one solution.

 mRNA holds several unique properties that are advantageous in the development of new therapies, including highly localized expression patterns, rapid development time, and a high degree of safety as a non-genome editing substrate for therapies.

Yeasen's custom mRNA production with superior purity, 3 month timeline.

Yeasen mRNA services can help to streamline the vaccine and drug development process and accelerate the translation of targets into practical therapies by helping to smooth the transition from early research and development to GMP mRNA manufacturing.

Request A Quote from Gentaur

Contact Our Expert Lieven Gevaert Bio-ir. RUG 1996

https://www.yeasenbio.com/pages/distributors

1. Superior mRNA Quality
2. Built-in CE test to guarantee mRNA quality
3. Guaranteed LNP particle size and uniformity
4. Comprehensive QC tests panel

​3 kits, totaling 35,000 EUR, you are eligible for a free demo unit installation. 

Schedule your installation at a time that’s convenient for you by selecting from the available slots below.

Terms:

• Purchase must be 3 or more kits.
• Total order must equal or exceed 35,000 EUR.

To book, please select a date and time above.

The SeekOneDD Single Cell 3' Transcriptome Kit offers a comprehensive solution for single-cell RNA sequencing, incorporating the SeekOneDD Chip S3, Gasket, Carrier Oil, SeekOneDD 3' Barcoded Beads, and all necessary reagents for amplification and library construction. It also includes the SeekSoul Tools software for single-cell data analysis. Leveraging advanced microfluidic technology, the kit facilitates single-cell isolation and capture using a water-in-oil system, while nucleic acid-modified Barcoded Beads ensure precise molecular labeling of RNA from individual cells. The resulting high-throughput single-cell transcriptome library is fully compatible with Illumina and MGI sequencers, making it ideal for applications in tumor research, immunology, cellular development, viral infection studies, drug discovery, and target screening.

SeekOne®scFAST-seq Releasing Meeting

SeekOne® Digital Droplet (SeekOne ® DD) Single Cell Whole Sequence Transcriptome Kit is a high-throughput single cell full sequence kit independently developed by Beijing Seek Biotechnology Co., Ltd. The kit breaks through the limitation that the traditional 3' or 5' transcriptome can only analyze the sequence at one end of the mRNA, and uses microfluidic digital droplets and Barcoded Beads conjugated with random primers to achieve random capture and detection of the transcriptome. The kit needs to be used with the company's self-developed SeekOne®® Digital Droplet System (SeekOne® DD) to complete the whole process from single-cell nucleic acid labeling to transcriptome library construction, and is equipped with single-cell data analysis software SeekSoul Tools to provide you with a one-stop single-cell transcriptome solution.

The SeekOne® DD Single Cell Whole Sequence Transcriptome Kit includes SeekOne® DD Chip S3 (Chip S3), Gasket, Carri-er Oil, SeekOne® DD Barcoded Beads (Barcoded Beads), amplification reagents, library construction reagents, and single-cell data analysis software (SeekSoul Tools).

A Single Cell Whole Transcriptome Sequencing Kit is a tool designed to analyze the full range of RNA transcripts from individual cells. Unlike bulk RNA sequencing, which pools RNA from many cells, single-cell transcriptomics allows researchers to explore the gene expression profiles of individual cells, revealing cellular heterogeneity, rare cell populations, and dynamic gene expression changes in tissues.

Here’s a breakdown of the kit’s components and features:

1. Single-Cell Isolation and Capture: The kit typically uses microfluidic systems (droplet-based or plate-based) to isolate individual cells. Each cell is captured in a small droplet or well, keeping it separate for further processing.
2. RNA Capture and Barcoding: Once isolated, RNA from each cell is captured and labeled with unique molecular identifiers (UMIs) or barcodes. This step ensures that the transcriptome from each cell is individually tagged, preventing cross-contamination between cells.
3. cDNA Synthesis and Amplification: The captured RNA is reverse-transcribed into complementary DNA (cDNA), which is then amplified to increase the quantity of material available for sequencing. This allows even small amounts of RNA to be detected and analyzed.
4. Library Construction: The kit includes reagents to prepare the amplified cDNA into sequencing libraries. These libraries are compatible with high-throughput sequencing platforms, such as Illumina or MGI, enabling large-scale parallel sequencing of thousands to millions of cells.
5. Whole Transcriptome Coverage: Unlike targeted approaches that focus on specific genes, a whole transcriptome kit sequences all expressed genes, giving a comprehensive view of cellular gene expression. This is essential for discovering novel transcripts, non-coding RNAs, and alternative splicing events.
6. Data Analysis: Many kits come with accompanying bioinformatics software or platforms, allowing researchers to analyze the high-dimensional data. These tools help identify differentially expressed genes, group cells into distinct types or states (like in cancer or immune studies), and explore complex biological pathways.

Applications:

• Tumor Research: Identifying heterogeneous cancer cell populations, including rare tumor stem cells and metastatic cells.
• Immunology: Profiling immune cells, understanding their roles in infection, autoimmunity, or therapeutic responses.
• Developmental Biology: Mapping cell lineage and differentiation pathways during development.
• Neuroscience: Investigating neuronal diversity and plasticity.
• Infectious Diseases: Studying host-pathogen interactions at the single-cell level.
• Drug Development: Exploring drug responses and resistance at a cellular resolution, allowing for more precise targeting in therapies.

​3 kits, totaling 35,000 EUR, you are eligible for a free demo unit installation. 

Schedule your installation at a time that’s convenient for you by selecting from the available slots below.

Terms:

• Purchase must be 3 or more kits.
• Total order must equal or exceed 35,000 EUR.

To book, please select a date and time above.

The SeekOneDD single-cell immunoassay kit is independently developed by Beijing Seek Biotechnology Co., Ltd., which is a product that realizes the acquisition of single-cell 5' transcriptome and immune group library sequence information based on microfluidic digital droplet technology. At the same time, the kit is equipped with SeekSoul Tools, a single-cell data analysis software independently developed by Cause, which can provide a one-stop single-cell 5' transcriptome and immunorepertoire solution.

The kit includes a single-cell 5' transcriptome kit and an immunobanking (BCR/TCR) kit, which can simultaneously obtain the full-length sequence of V(D)J, the pairing information of the α chain (heavy chain) and β chain (light chain) of TCR (BCR) and the corresponding transcriptome information, which can be used for tumor immune microenvironment exploration, immune cell regulatory mechanism exploration, immunotherapy target screening, infectious disease research and vaccine screening.

Single cell capture and labeling process

Library construction process

cDNA Reverse Transcription and Enrichment
This section represents the process of reverse transcription where mRNA is converted into cDNA and enriched for further processing.

Immunoglobulin Library
The visual here depicts the steps involved in constructing an immune repertoire library, including primer binding and amplification processes.

5' Transcriptome Library
This section shows the preparation of the 5' transcriptome library, starting with the cDNA and then proceeding through additional steps for library preparation, indexing, and sequencing readiness.

​3 kits, totaling 35,000 EUR, you are eligible for a free demo unit installation. 

Schedule your installation at a time that’s convenient for you by selecting from the available slots below.

Terms:

• Purchase must be 3 or more kits.
• Total order must equal or exceed 35,000 EUR.

To book, please select a date and time above.